Studies on Stability of 5′-Ribonucleotides

Reversed-phase liquid chromatographic separation of 3',5'-cyclic ribonucleotides.

A rapid, reversed-phase "high-performance" liquid-chromatographic separation of the five naturally occurring cyclic ribonucleotides is described. The separation, optimized for the measurement of these compounds in biological samples, is short (25 min), sensitive (50-100 pmol), and requires no sample pre-concentration steps. We also report an alternative isocratic elution mode, optimized for a r...

Studies on 5-hydroxyuridine.

postnatal studies of bats (pipistrellus kuhlii and miniopterus schreibersii) & histomorphology and histochemistry studies of organs and diseases of (neurergus microspilotus and n. kaiseri)

1. to determine whether difference in birth body mass influenced growth performance in pipistrellus kuhlii we studied a total of 12 captive-born neonates. bats were assigned to two body mass groups: light birth body mass (lbw: 0.89 ± 0.05, n=8) and heavy birth body mass (hbw: 1.35 ± 0.08, n=4). heavier body mass at birth was associated with rapid postnatal growth (body mass and forearm length) ...

Ribonucleotides of RNA: separation by chromatography on sheets of diethylaminoethylcellulose.

The four main ribonucleotides of RNA can be separated on diethylaminoethylcellulose paper sheets, with results comparable to column chromatography, by irrigation with 0.05M formic acid for 2 to 3 hours, and then reversing the direction of irrigation with 4M formic acid.

Syntheses of some fluorescent ribonucleotides.

Synthesis of guanosine 3'-phosphodiesters of 1, N6-ethenoadenosine-2-sulfonate or 1, N6-etheno-2-azaadenosine can be done readily with ribonuclease N1. There is a 10-fold difference in quantum yield between the phosphodiesters and the starting fluorescent nucleosides. This difference provides the basis for the development of a fluorescent assay for nuclease.